Fig. 2. Molecular analysis of the Gug locus. (A) The genomic region of Gug with the two P-element insertions and the deletion Df(3L)Gug³⁵. The restriction enzyme sites of EcoRI, SalI and HindIII are shown. (B) Structure of the cDNA of Gug. The black boxes indicate the coding sequence. (C) Northern analysis: a 9.0 kb zygotic and a 8.0 kb maternal-specific transcript are detected. L, third instar larva; F, female. (D-F) In situ detection of Gug transcripts in a blastoderm (D) and germband extended (E) embryos and leg disc (F) associated with the larval central nervous system (cns, arrow). (D,E) Anterior is towards the left and ventral is towards the bottom. Note that Gug is not detected in the amnioserosa (as) in E and is concentrated in the hemispheres of the brain (arrow, F). In the leg disc, v, d and di indicate the ventral, dorsal and distal regions, respectively.