Fig. 8. Gug activity is required for regulation of teashirt (tsh). Clones of Gug^– cells in imaginal leg discs, detected by the absence of the Myc epitope. (A) Clone, showing a ventral outgrowth in the proximal, ventral region. Overlapping expression of Tsh and Myc is shown in yellow. Tsh is absent in ventral Gug^– cells (white line) and in wild-type cells (red line) adjacent to the clones. In dorsal clones, Tsh is expressed normally as shown by the green staining (green line). (B) Tsh (green) and Dll (red) expression in a late third instar disc showing a small secondary axis. Distalisation is indicated by the ectopic Dll expression in this new axis (arrow). Note that this disc is shown at twice the magnification. (C) Distal (ventrally and dorsally) clone marked by the absence of Myc (red); Dll (green) is expressed normally in the clone. (D) Wg-lacZ expression (green and middle panel) is reduced in a small ventral outgrowth (arrow) induced by a Gug^– clone (absence of the red staining; right panel). (E) Wg-lacZ expression (green and middle panel) is normal in a distal ventral clone (absence of the red staining and right panel). Note that there must be at least two clones, one in the anterior and another in the posterior compartment. (F) Dpp-lacZ (green and middle panel) is expressed weakly in some Gug^– cells in a ventral outgrowth (arrow; right panel).