Fig. 3. Dichaete is expressed in the medial and intermediate neuroectodermal columns and in lateral NBs. Low- (A) and high- (B-D) magnification ventral views of wild-type stage 8 (A,B) or stage 11 (C,D) embryos labeled for Dichaete (A), Dichaete (green) and msh (red) (B), or Dichaete (brown) and engrailed (blue) (C,D). (A) In wild-type embryos, Dichaete is expressed in the ventral region of the neuroectoderm. (B) The lateral limit of Dichaete expression abuts precisely the medial limit of msh expression in the lateral column (arrows). (C) In early stage 11 embryos, Dichaete expression persists in the medial and intermediate columns; however, lateral column NB 7-4 now expresses Dichaete (arrowhead). (D) In late stage 11 embryos two additional lateral column NBs (NB 2-5 and NB5-6) express Dichaete (arrows). Anterior is towards the left and the line indicates the ventral midline.