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Fig. 4. Expression patterns of dpy-11 and subcellular localization of its product. (C,E,G) Nomarski images of D,F,H, respectively. (A) dpy-11 promoter-driven gfp reporter with a nuclear localization signal in L3 larva. (B) Cytosolic gfp reporter in L4 larva showed no seam cell expression (arrowhead). (C,D) The DPY-11 tagged with GFP at the C-terminus (pd11{Delta}CTGFP) presented a heterogeneous cytosolic expression pattern in the hypodermis around the vulva. (E,F) The fusion transgene encoding full-length DPY-11 with a GFP tagged between the transmembrane domain and the C-terminus (pd11AGFPC) presented a heterogeneous cytosolic expression pattern in the body hypodermis. (G,H) In gastrulating embryos, cytosolic expression (pd11{Delta}CTGFP) was detected in unfused hypodermal cells (arrows) with membrane association detected in some dying cells (arrowheads). Scale bars: 100 µm (A,B); 10 µm (C-H).