Fig. 5. kal-1 mutants show neuronal growth defects. Epifluorescence images of neurons of adult worms, harboring the reporter plasmid GB102 on an extrachromosomal transgenic array. In all panels, anterior is towards the left. (A,D) control; (B,C,E) kal-1 mutants. (A-C) The EF3 male-specific neuron is visible in these worms because of GFP expression from the transgene. In mutant males, the EF3 dendrite presents an extra-branching with the formation of a second dendrite running in the same direction and parallel to the normal one. (D-F) A neuron of the head, RIC, is visible in these worms because of GFP expression from the transgene. In mutant worms overexpressing CeKAL the axon presents an extra-branching with the formation of a spike running parallel but in the opposite direction to the normal path. In F, the trace of the neuron is drawn from micrograph D, and the broken line represents the extrabranching visible in E. Arrows indicate extra-branching in the mutants.