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Fig. 2. Gene expression in animal caps treated with activin or eFGF. RNA was extracted at stages 10, 11, 11.5 and 12 from whole embryos, untreated animal cap explants and animal cap explants treated with eFGF or activin. Total RNA (7 µg) from each stage was analysed by RNAase protection for expression of the marker genes XmyoD, Xbra and the loading control ODC.