Fig. 3. XmyoD induction in eFGF and CHX treated animal caps. (A) RNA was extracted at stage 13 from whole embryos, animal caps in the presence or absence of CHX, and animal caps cultured with eFGF in the presence or absence of CHX. Total RNA (3 µg) was analysed by RNAase protection for the expression of the marker genes XmyoD, Xbra and for the loading control ODC. (B) RNA was extracted at stage 18 from whole embryos, animal caps in the presence or absence of CHX, and animal caps cultured with eFGF in the presence or absence of CHX. Total RNA (10 µg) was analysed by RNAase protection for the expression of the marker gene actin and for the loading control ODC. (C) RNA was extracted at stages 11, 13 and 18 from whole embryos and animal caps cultured in the presence or absence of CHX. Total RNA (10 µg) was analysed by RNAase protection for the marker genes XmyoD, Xbra and the loading control ODC.