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Fig. 6. Activin induces eFGF expression in the presence of CHX. RNA was extracted at stage 11 from whole embryos, untreated animal cap explants, CHX treated animal cap explants and caps treated with activin in the presence or absence of CHX. Total RNA (7 µg) was assayed by RNAase protection for expression of eFGF, the mesodermal marker Xbra and the loading control ODC.