Fig. 3. Slit protein levels are reduced in lola mutant embryos. A collection of lola mutant embryos and their non-mutant siblings was fixed, stained with anti-Slit and visualized by peroxidase histochemistry. (A) Wild type; (B) lola1A4. Identical camera settings were used for both embryos shown. Lateral view of the CNS midline of a late stage 16 embryo is shown in each panel; midline glial cells are prominently stained (arrows). Note that Slit immunoreactivity is present, but at reduced level, in glial cells in each segment in the mutant. Anterior is towards the left.