Fig. 7. Cell death and cell proliferation in the Pax6 mutant hindbrain. Sections at r7 level of E12.5 wild-type (A) and Pax6 mutant (C), and cultured wild-type (B) and Pax6 mutant (D) embryos. (E,F) Comparison of TUNEL- (E) and BrdU- (F) positive cells per section within the ventral region, including the Dbx1 domain using 10 sections from three embryos. (E) There is no significant difference in the number of apoptotic cells between the two groups. (B,D) Pulse labelling of BrdU was performed for embryos cultured for 20 minutes and BrdU labelled cells were detected with anti-BrdU antibody. The ratio of BrdU labelled cells is slightly increased in the Pax6 mutant (t-test; *P<0.001).