Fig. 3. Ras activity inhibits the organizing capacity of the DV compartment boundary. (A-E) vn^– (A,B,E) and wild-type (C,D) discs containing clones ectopically expressing either Ras^V12 (A,C,D), Rho (B) or Ap (E) monitored for the expression of Ap (red in A,C,E), ap-lacZ (red in B,D), Wg (blue in B), wg-lacZ (blue in C) and Vg (blue in D). Clones were induced during the first instar and are marked by presence (A,C,D) or absence (B,E) of GFP expression (green). Clones in B appear in red. Overlap of red and green signals appear in yellow (B-D). (A) vn mutant disc containing a Ras^V12-expressing clone that induces ap expression autonomously, but fails to exert a non-autonomous effect on proliferation of neighboring vn mutant cells. The inset shows a vn mutant disc at the same magnification. (B) Rho-expressing clones (arrows) that exert a non-autonomous effect on both ap-lacZ expression and proliferation of vn mutant cells. The ap^ON-ap^OFF interface is associated with Wg expression. Note that the disc is shown at 1.3x the magnification used in A. (C) Wild-type disc containing a Ras^V12-expressing clone that autonomously expresses Ap, but does not induce wg-lacZ expression along the clone border. (D) Clones of Ras^V12-expressing cells in a wild-type disc autonomously induce ap-lacZ and repress Vg expression. (E) vn mutant disc containing an Ap-expressing clone that non-autonomously rescues the proliferation of the wing blade primordium. The disc is shown at the same magnification as the disc in A.