Fig. 1. Amino acid sequence comparison of Pax6A and Pax6B from D. japonica and G. tigrina. (A) Multiple alignment of DjPax6A, GtPax6A, and DjPax6B, GtPax6B sequences. Amino acids that are identical both in Pax6A and Pax6B are shown in blue; amino acids conserved between DjPax6A and GtPax6A are indicated in green; amino acids conserved between DjPax6B and GtPax6B are indicated in red; differing amino acids are shown in black. The paired domain, the conserved motif found in the linker region, and the homeodomain are boxed. The missing sequence in the 5' end of GtPax6A is indicated by dots; the introduced gaps are indicated by dashes. (B,C) Sequence comparison of the paired domain (B), and the homeodomain (C) of the planarian Pax6 proteins to the Pax6 paired domain and homeodomain of different species. Only Homo sapiens and Mus musculus Pax6 are shown for vertebrates. Drosophila eyless and toy are included as invertebrate Pax6. The shaded bars indicate Pax6-specific amino acids. Percentages of sequence identity (%I), determined by comparison with Homo sapiens and Mus musculus Pax6, are indicated at the end of each line. The structure of paired domain and homeodomain are shown on the top in B and C, respectively. Identical amino acids are indicated by dots. The incomplete sequence of GtPax6A is indicated by a dashed line. Non conserved amino acid residues are indicated in bold. The homeodomain flanking regions are shown in C.