Fig. 10. Expression of GtSix-1 and Gtops in regenerating G. tigrina after injection with Pax6A/Pax6B dsRNA mixture, visualized by whole-mount in situ hybridization. Dorsal view of head regenerating fragments after 3, 6 and 9 days from cutting. (A-C) Water-injected controls hybridized with Gtsix-1. (D-F) GtPax6A/GtPax6B dsRNA mixture-injected organisms hybridized with GtSix1. (G-I) GtPax6A/GtPax6B dsRNA mixture-injected organisms hybridized with Gtops. (A,D) A faint GtSix1 hybridization signal is visible after 3 days of regeneration both in the controls and in injected animals. Later on, GtSix1 mRNA is clearly visualized at the eye level. No differences are detected between controls (B,C) and injected animals (E,F). (G-I) A normal expression pattern of Gtops mRNA can also be detected in animals injected with GtPax6A/GtPax6B dsRNA. Scale bars: 0.5 mm.