Fig. 6. Purified Bdnf^–/– granule cells have defects in motility that are rescued by BDNF. Purified wild-type and Bdnf^–/– granule cells were grown for 24 hours in Boyden chambers without (control) or with (+BDNF) 30 ng/ml BDNF in both chambers. Granule cells that migrated through the porous membrane into the lower chamber were directly quantified by light microscopy. Purified Bdnf^–/– granule cells exhibited reduced migration in the control medium that could be rescued to wild-type levels by the addition of BDNF. *P<0.05 (Student’s t-test) for wild-type versus Bdnf^–/– cells in control conditions. White bars, wild-type animals; black bars, Bdnf^–/– animals. Pooled results from three separate experiments are shown.