Fig. 5. Changes in the visual system and the LNs during pupation. Whole mounts of w; pdf-gal4 UAS-gfp pupal brains doubly stained with anti-chaoptin (red) for the visual system and GFP (green) for the LN_vs. Similar results were obtained with both gal1118-driven GFP labeling and anti-PDF labeling of the LN_vs (not shown). However, most of the experiments were performed with pdf-gal4-driven GFP labeling, because it remained restricted to the s-LN_vs for the longest developmental time, thus ensuring that the arborization observed up to 32 hours APF indeed originated from the s-LN_vs and not from the l-LN_vs. Time APF is indicated in hours. Thin arrows indicate the BN that is detected up to 8 hours APF (A-C). (D,E) Thick arrows indicate the reduced dendritic arborization of the LNs at 16 hours (D) and 32 hours (E) APF. Arrowheads show the newly appeared chaoptin-expressing fiber (F-H). G,H are the same sample, but H shows only chaoptin staining for better visualization. APP, adult photoreceptors projections; BN, Bolwig nerve; LNs, lateral neurons; DP, dorsal projections of LNs; l-LN_vs, large ventral lateral neurons; s-LN_vs, small ventral lateral neurons; M, medulla. Scale bar: 10 µm.