Fig. 3. Tail hypodermal cell defects in tlp-1 animals. Adherens junctions are labeled by JAM-1::GFP and shown in green. (A,C,E) Wild type. (A) Late L4 male, left-ventral view at a stage when the ray cells begin to cluster and the tail tip cells fuse to form the tail tip syncytium. Cell fusions indicated by punctate adherens staining. (C,E) Adult hermaphrodites, left-ventral and left views, respectively. Note that hyp11 is located dorsally above hyp8. (B,D,F) tlp-1(bx85). (B) Male, ventral view, same stage as that in A, but fusion of tail tip cells has not yet occurred. In addition, only two T-ray primordia are visible on the left side of this animal and all appear to be missing on the right side. (D,F) Hermaphrodites, left view. (D) hyp8 and hyp11 do not appear to be bounded by adherens junctions and could have fused with hyp7; adherens ‘outlines’ of phasmids are not formed or are formed aberrantly. (F) Although the adherens junctions of the phasmid look normal, the tail tip cells are not organized as in wild type, in that at least one extra cell appears in the group of tail tip cells. Scale bar: 20 µm.