Fig. 6. tlp-1 expression responds to the LIN-44 signaling pathway. (A) tlp-1::gfp expression in a lin-44 animal. Fluorescence (upper) and DIC optics (lower). Scale bar: 10 µm. (B) Cell lineages of the T cell in lin-44 and lin-44; tlp-1(mh17) hermaphrodites. We analyzed T cell lineages on both sides of four lin-44; tlp-1(mh17) double mutants. Two had the T cell lineages similar to that of tlp-1 single mutant (Fig. 1). One had the T cell division pattern shown in the upper right corner. One had the T cell division pattern shown third in the lower left corner and four had the T cell division pattern shown in the lower right corner; however, in one, T.app cell divided to generate two neural cells and T.pa divided to four hypodermal cells, and in another T.pa divided to generate four hypodermal cells. Circular arrows indicate positions of inferred polarity reversals. (C) Cell lineages of the T cell in lin-17 and lin-17(n671); tlp-1(mh17) hermaphrodites. lin-17 hermaphrodite T cell lineage is shown on the left, in which T.xx divide once or twice to generate two or four hypodermal cells (Sternberg and Horvitz, 1988). We analyzed T cells lineages in six lin-17(n761); tlp-1(mh17) double mutants. In three, there were no further T.xx cell divisions. In six, T.ap divided either once (two lineages) or twice (four lineages) to generate two or four hypodermal cells, respectively. In two, T.pa divided twice to generate four hypodermal cells, however in one of these T.ap also divided twice to generate four hypodermal cells. In the last one, both T.ap and T.pp divided once to generate two hypodermal cells. Arrows indicate that a particular cell was sometimes observed to divide in a subset of the lineages.