Fig. 5. Slug overexpression increases the migratory neural crest population in the cranial region of the chick. Control (A) and electroporated sides (B) of the same whole-mounted embryo hybridised with rhoB 24 hours after electroporation with Slug (stage 15). In B, observe the higher number of rhoB positive cells in the crest cells migrating from the forebrain, midbrain and hindbrain regions (arrowheads). (C,D) Another Slug electroporated embryo double labelled for rhoB and HNK-1 at stage 17. (E) A section taken from this embryo at the different levels indicated by the dotted lines in C and D allows the visualization of different crest populations that are increased in the electroporated side (top in this picture). (F,G) High power views of the boxed areas in E. Black arrowheads, rhoB-positive/HNK-1-positive cells; white arrowheads, rhoB positive cells; black arrows, HNK-1-positive cells.