Fig. 3. Tissue specific analysis of Igf2 expression. (A) Northern analysis of Igf2 and H19 in neonatal (P1) tissues following paternal transmission of Mnt and the corresponding wild-type littermates. (B) Histogram of relative Igf2 expression levels normalised against Gapdh. Error bars show the standard deviations when multiple samples were analysed: tongue 35% (n=2 wild type, n=2 Mnt^P), liver 110% (n=5 wild type, n=6 Mnt^P), intestine 71% (n=4 wild type, n=5 Mnt^P), muscle 62%% (n=1 wild type, n=2 Mnt^P). (C) In situ hybridisation analysis of Igf2 expression in E14 Mnt^P embryos and placentae. Images were captured with standardised exposure times, degree of illumination and level of magnification. Choroid plexus (cp), tongue (t), heart (h), lung (lu), liver (li), intestine (i) and intercostal muscle (im). Note the difference in embryonic and placental sizes between Mnt^P and wild type. (D) Higher magnification of some organs. Note the reduced levels (apparently cell-type specific) observed in the intestine, lung and tongue. (E) Cell-type specific expression within the intestine. Expression in Mnt^P is retained in the endodermal epithelial lining (e) but lost in the mesodermal muscular layer (m) of the intestine. (F) Cell-type specific expression within the lung. Igf2 expression in Mnt^P is only retained in those bronchi with closed lumen (c) while it is lost in the open bronchi (o) and mesenchyme.