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Fig. 6. Transgene expression analysis to identify mesodermal enhancers for Igf2. (A) The two transgenes microinjected for transient expression analysis. Restriction sites NotI (N) and SalI (S) cut within the bluescript vector to excise +gh transgene. NotI and XhoI (X) digest excises –gh transgene. 5' H19 constitutes nucleotides 4700-5523 (H19 promoter), while 3' H19 constitutes nucleotides 9511-36040 (GenBank Accession Number, AF049091). (B) lacZ expression analysis in +gh-positive E14 embryo. (C) lacZ expression analysis in –gh-positive E14 embryo. Organs indicated are liver (li), intestine (i), tongue (t), heart (h) and lung (lu).