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Fig. 7. Whole-mount antibody staining of stage 40 Xanx-4 MO-injected embryos identifies a tubule phenotype. One-cell stage Xenopus embryos were injected with 10 ng of Xanx-4 MO, cultured to stage 40 and subjected to whole-mount antibody staining with pronephric tubule specific antibody 3G8. (A) Normal uninjected control embryo. (B) Embryo injected with Xanx-4 MO shows shortened, enlarged tubule phenotype.