Fig. 1. Generation of Rho GDI transgenic founder lines. (A) Schematic diagram of transgenic vector showing insertion of Rho GDI cDNA under the MHC promoter and locations of the primers used for genotyping by PCR. (B,C) Representative Southern blot and PCR screening of nontransgenic (NTG) and transgenic (TG) mice using tail DNA. ß-casein was also amplified as a control. (D) Quantitative DNA dot blot analysis was performed as described in the Materials and Methods, and identified six transgenic founder lines (L1, L2, M1, M2, H1 and H2) with copy number ranging from 1 to 20.