Fig. 6. Subcellular localization of p90Rsk2 in mouse spermatocytes. The panels on the left show the immunofluorescence analysis of control (top) or OA-treated (6 hours with 0.5 µM, bottom) cells. Immunostaining was carried out using the anti-p90Rsk2 antibody and a rhodamine-conjugated secondary antibody as described in the Materials and Methods. Cells were also stained with Hoechst 3332 to detect chromatin (right panels).