Development -- Kozlova and Thummel 129 (7): 1739 Figure IG7

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Fig. 7. GAL4-USP activation is significantly reduced in an EcR mutant background. GAL4-USP activation was assayed in yw; EcR^M554fs/CyO, y⁺; hs-GAL4-USP/UAS-nlacZ or yw; EcR^A483T/CyO, y⁺; hs-GAL4-USP/UAS-nlacZ animals (control) staged in parallel with yw; EcR^A483T/EcR^M554fs; hs-GAL4-USP/UAS-nlacZ animals (EcR-ts) at the onset of metamorphosis. The cross was shifted to 29°C for 24 hours before heat treatment and histochemical staining. GAL4-USP activation is greatly reduced in the midgut, muscles and fat body in the EcR-ts mutant (D-F) compared to controls (A-C).