Fig. 8. GAL4-EcR and GAL4-USP expression leads to stage-specific lethality. The graph shows percentage viability after a single 30 minute heat treatment at 37°C of third instar larvae between 8-0 hours before puparium formation (late L3) or early pupae 12-20 hours after puparium formation. Five different stocks were tested: a w¹¹¹⁸ control (gray bars), two hs-GAL4-EcR lines outcrossed to w¹¹¹⁸ (blue bars), and two hs-GAL4-USP lines outcrossed to w¹¹¹⁸ (red bars) (n=120-150 for each line).