Fig. 3. Inv-Cldn6 skin. (A) As a consequence of the transgene, the skin of Inv-Cldn6 neonates is red and shiny in appearance and becomes dehydrated and cracked within a few hours of life. (B,C) Newborn backskin samples from transgenic and wild-type mice were fixed with Bouin’s solution, processed and embedded in paraffin and sections were stained with Haematoxylin/Phloxine/Safranin. The transgenic epidermis is highly disorganised with gaps apparent throughout in addition to non-uniform basal cells. (D,E) It is also apparent that there is a dramatic decrease in keratohyalin granular cells and that the stratum corneum is thicker and often fragmented in the transgenic samples. (F,G) There is also an obvious reduction in the occurrence of subcutaneous fat pads resulting in the overall reduction in the thickness of transgenic skin.