Development -- Clotman et al. 129 (8): 1819 Figure IG5

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Fig. 5. Expression of liver-enriched transcription factors in control (+/+ and +/–) and in Hnf6^–/– mice. The expression levels were assessed by semi-quantitative RT-PCR on liver RNA. The data are representative of three independent experiments. (A) At E14.5, the expression levels of Hnf3ß, Hnf3 ${gamma}$ and Hnf4 ${alpha}$ are not affected by inactivation of the Hnf6 gene. The expression levels of Hnf1 ${alpha}$ and Hnf3 ${alpha}$ are slightly increased in Hnf6^–/– fetuses, whereas the two splicing isoforms of Hnf1ß (Hnf1ß-A and Hnf1ß-B) are strongly downregulated. At P3, the expression levels of Hnf3ß, Hnf3 ${gamma}$ and Hnf4 ${alpha}$ are not affected, the expression of Hnf1ß has normalized and that of Hnf1 ${alpha}$ and Hnf3 ${alpha}$ is still upregulated. (B) At E12.5, the expression of the two splicing isoforms of Hnf1ß is strongly downregulated in the Hnf6^–/– livers, as assessed with Tbp as a reference.