Fig. 6. Analysis of osteoclast markers demonstrates reduced numbers of osteoclasts in the perichondrium of VEGF^120/120 mice. MMP9 expression in wild-type (A) radius and ulna (arrowheads) is much more extensive than in VEGF^120/120 (B) radius (arrowheads). This is consistent with the results of TRAP staining. Numerous TRAP-positive cells are seen in the perichondrium of wild-type radius (C, arrowheads), in contrast to the almost complete absence of TRAP-positive cells in VEGF^120/120 radius (D). Inserts show TRAP-positive cells at a higher magnification.