Fig. 3. The effect of testosterone on SM in P0 female UGTs grown in vitro. (A,B) SM distribution in P0 female UGT grown in vitro for six days in the absence of testosterone; (C,D) a similar culture to which 1x10^–8 M testosterone has been added. Scale bars shown represent 100 µm. (E) UGT from a P3 female. (F) A graph of SM thickness in response to testosterone (±s.d., five experiments, 628 measurements). Testosterone treatment led to a 2.4-fold reduction in SM thickness.