Fig. 5. Later differentiation of mesodermal cells following manipulation of LvDelta levels. (A-C) Pigment cells; (D-F) SMC2 staining (blastocoelar cells are indicated by arrowheads; there is background staining in the ectoderm and endoderm, showing the outline of the larva and the midgut, respectively); (G-I) anti-myosin staining showing muscle fibers. (A,D,G) Control pluteus larvae showing normal numbers of pigment cells, blastocoelar cells and muscle fibers. (B,E,H) Following injection of LvDelta morpholino, the resulting larvae have few pigment cells, blastocoelar cells and muscle fibers. (C,F,I) Following injection of LvDelta mRNA, increased numbers of pigment cells, clusters of blastocoelar cells and increased muscle fibers are apparent.