Fig. 3. CD9-eGFP fluorescence observed for CD9^–/– oocytes injected with either wild-type or F174A mutant mouse CD9-eGFP mRNA. (A) Fluorescence observed in CD9^–/– eggs injected with wild-type CD9-eGFP mRNA. (B) Fluorescence observed in CD9^–/– eggs injected with F174A mutant CD9-eGFP mRNA. In A and B, both intracellular and surface fluorescence are observed. (C) Fluorescence observed in uninjected CD9^–/– eggs. (D-F) Phase contrast images of A-C, respectively. Fluorescence levels were somewhat variable within each group of eggs injected with the identical amount and type of mRNA.