Fig. 3. Hematopoietic colony formation in EB6 cells from the different CD34 and CD41 populations. Various EB6 cell fractions were sorted by flow cytometry according to CD34 and CD41 expression and cells were plated in methylcellulose for colony assays. Control 1 (C1) is the unfractionated cell population. Control 2 (C2) corresponds to EB6 cells sorted with the side scatter and forward scatter gating used for CD41 purification (n=6). Results are expressed as percentages (number of progenitors for 100 plated cells).