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Fig. 5. Identification of primitive erythropoiesis in the different CD34 and CD41 populations purified from EB6 and yolk sac (YS) cells. Erythroid colonies derived from primitive erythropoiesis (PE) were identified on an inverted microscope. They were observed in both the CD41+ and CD41 cell fractions, but essentially in CD34CD41+ cells. Results are expressed in percentages (ratio of the number of primitive erythroid colonies in each fraction to the total number of primitive erythroid colonies).