Fig. 8. Cell sorting of different CD34 and CD41 populations from yolk sac (YS), AGM, fetal liver (FL) and bone marrow (BM) cells. Double staining was performed with an FITC-conjugated anti-CD41 MoAb and a biotinylated anti-CD34 MoAb with streptavidin-R-PE used as the secondary reagent. For yolk sac, fetal liver and bone marrow cells, four cell subsets represent CD34⁺CD41⁺, CD34^–CD41⁺, CD34⁺CD41^– and CD34^–CD41^– were sorted in the R1, R2, R3 and R4 gates, respectively. For AGM, three cell subsets, the entire CD41⁺ cell population, the CD34⁺CD41^– and CD34^–CD41^– cell fractions were sorted, corresponding to the R1, R3 and R4 gates, respectively.