Fig. 1. (A) Sequence analysis (GenBank Accession Number, AF391125). The runt domain (RD) is in red. The region encompassed by two perpendicular bars indicates the longer template, and the region in green, the shorter template for in situ probes. The runx1-MO1 and runx1-MO2 binding sites are indicated by horizontal lines. (B) Percentage amino acid identity of zebrafish Runx1 when compared with the Xenopus, mouse and human orthologs. Overall identity is indicated in black, and identity within the RD in red. (C) Phylogenetic analysis of runx1. The GenBank Accession Numbers of the genes included in the analysis are: human RUNX1, L34598; mouse Runx1, D13802; Xenopus runx1, AF035446; human RUNX2, XM_004126; mouse Runx2, AF010284; human RUNX3, X79550; mouse Runx3, AF155880; and zebrafish runx3 (runxb transcript 1), AB043788. Bootstrap support values are given in the nodes. (D) The runx1 locus maps to the upper portion of zebrafish LG1 in a region, showing conserved syntenies with a portion of human chromosome 21. Distances on LG1 are in centiMorgans (cM) (the entire chromosome is 122 cM long), and distances on Hsa21 are in megabases (Mb). Abbreviations: gart (AF257743; D. B. Slavov and K. Gardiner, unpublished), ortholog of GART in human; app (AF257742; D. B. Slavov and K. Gardiner, unpublished), ortholog of APP in human; mx1 (AW202878; Washington University Zebrafish EST Project 1998, unpublished), apparent ortholog of MX1 in human (Woods et al., 2000); other markers of the form Z4593 are from Shimoda et al. (Shimoda et al., 1999) and http://zebrafish.mgh.harvard.edu/.