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Fig. 4. Effects of runx1 expression in cloche (A,B,D-F) and wild-type (C) zebrafish embryos at 48 hpf. Lateral views of anterior regions (A-C) and whole embryos (D-F), anterior towards the left. (A-C) Morphology and (D-F) expression of hbbe3 (globin). (A,B) Blood in the trunk of clo embryo injected with runx1 (arrowhead) compared with uninjected clo. Arrows indicate dilated heart. (C) Ectopic blood (arrowhead). (D-F) hbbe3 (globin) expression on the yolk (arrowhead) and in the trunk (arrow) in clo injected with runx1 compared with uninjected clo and wild type.