Fig. 8. Neurological effects of Runx1 depletion. Morphological phenotype (A-D) and effect on HNK-1 expression (E,F) at 48 hpf. (A,B) Lateral and (E,F) dorsal views of head regions. (C,D) Lateral views of tail regions; anterior towards the left. Controls are indicated. (A,B) Enlarged ventricular space (arrowhead) and supernumerary otoliths (arrow) in runx1-mo embryo compared with normal. (C,D) Interrupted notochord (arrow indicates its anterior and arrowhead indicates posterior region in runx1-mo embryo). The red arrow in C indicates an accumulation of blood cells. In control, notochord (black arrow) and caudal vessels (red arrow) are shown. (E,F) Reduction in trigeminal descending (arrow) and Rohon-Beard ascending (arrowhead) central axons and abnormal localization of Rohon-Beard cells (brown arrow).