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Fig. 5. Cx43{alpha}1 expression in the proepicardial organ. (A) E9.5 embryo whole mount immunostained with a Cx43{alpha}1 antibody was embedded and sectioned. (A) Phase contrast image of a section which includes the PEO. The boxed region is magnified in B,C. Note the dendritic mesenchymal cell morphology (B, phase contrast). Cx43{alpha}1 immunostaining (C, darkfield) show punctate and long profiles of gap junction contacts between cell processes (arrows in B,C).