(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)


Fig. 6. Expression of Cx43{alpha}1 gap junctions in the epicardium of the fetal mouse heart. (A,B) Immunostaining shows Cx43{alpha}1 expression in the compact layer of the ventricular myocardium (myocard), in the ventricular trabeculae (trab) and in the epicardium (white arrows) in the interventricular sulcus of an E12.5 mouse heart. Cx43{alpha}1 expression is depicted in purple. The green background is autofluorescence recorded in the FITC (green) channel to delineate the myocardium and epicardium. Cx43{alpha}1 expression in the epicardium is comparable with that of the compact myocardium, while expression in the trabeculae is much higher. Cx43{alpha}1 expression in the ventricular myocardium and epicardium (white arrows) is shown at higher magnification in B. (C,D). Immunostaining with a cytokeratin antibody revealed abundant cytokeratin (blue) expression in the epicardium of Cx43{alpha}1+/– (C) and Cx43{alpha}1–/– (D) hearts.