Fig. 2. Decreased neuronal apoptosis in Lc/+; tPA^–/– cerebella at P12. Cellular apoptosis was detected using the TUNEL assay as described in the Materials and Methods. (A) TUNEL assay. Anti-fluorescein antibody conjugated with peroxidase (POD) was used to analyze the section under light microscope. Notice that more TUNEL-positive cells were detected in the Lc/+ cerebella than in the Lc/+; tPA^–/– cerebella. Scale bar: 20 µm. (B) Quantification of apoptotic neurons in cerebellum of wild-type, Lc/+ and Lc/+; tPA^–/– mice at P12. The data presented are mean±s.e.m. (n=6 mice). *P<0.05 by ANOVA.