Fig. 4. Secondary death of granule cells is decreased in Lurcher mice with tPA deficiency. Sagittal cerebella sections of wild-type, tPA^–/–, Lc/+, and Lc/+; tPA^–/– mice at P12 and P30 were stained with Cresyl Violet. At P12, both Lc/+; tPA^–/– and wild-type cerebella showed normal lobular patterns, well-developed folia and densely stained granule layers. By contrast, the Lc/+ cerebella were greatly reduced in size, with poorly formed folia and sparse granule cells. By P30, although Lc/+; tPA^–/– cerebella revealed atrophic folia, more granule cells were still detected there than in the Lc/+ cerebella. Scale bars: 100 µm (top); 20 µm (bottom).