Fig. 6. Caspase 8 activation is decreased in Lurcher mice that lack tPA. (A) Western blotting was performed for wild-type (wt), Lc/+, and Lc/+; tPA^–/– mice at P12 and P30, using either rabbit polyclonal caspase 8 antibody (1:500 dilution, reacts with both the p18 subunit and precursor of caspase 8). (B) Quantification of caspase 8 activation by western blot analysis. The activated caspase 8-specific bands were quantitated using a fluorimager, as described in the Materials and Methods. Note that the decreased caspase 8 activation (18 kDa band) in the Lc/+; tPA^–/– cerebellum was detected at both P12 and P30. The data presented are from four different experiments. (C) Coomassie Blue staining to show protein loading. *P<0.001 by ANOVA.