Fig. 2. Expression of plx-1::egfp in him-5; ncEx[plx-1::egfp, rol-6(su1006)]. The animals are shown with anterior towards the left. (A) A lateral view of the left side of a third larval-stage male tail at about 30 hours after hatching. GFP expression is observed in R(n) cells (arrows). (C) An early to mid-L4 stage male at about 35 hours after hatching. The ray precursor clusters (arrows) express GFP. (B,D) The corresponding DIC images shown in A,C, respectively. (E) A lateral view of seam cells at L4 stage. Both parental seam cells (arrows) and a daughter cell (arrowhead) express GFP. (F) A ventral view at early L3 stage. GFP expression is observed in ventral cord motoneurons (small arrows) and seam cells (an arrowhead). All vulval precursor cells (some are indicated with arrows) aligned along the ventral midline also express GFP. Scale bars: 20 µm.