Fig. 4. Displacement of ray precursors in plx-1 mutants. Cell boundaries of ray precursor clusters were visualized in wild-type (A,C) and plx-1(nc37) (B,D) animals with jam-1::GFP. All animals contain him-5. (B) At the early L4 stage, ray 1 precursor cluster, processes are localized to the junction site between R1.p, R2.p and the surrounding hypodermal syncytium (hyp7). (A,B) R1.p of a plx-1(nc37) animal is abnormally small (B) compared with that of the control animal (A). In the plx-1 mutant at the mid L4 stage (D), the position of the ray 1 precursor cluster (1) is shifted anteriodorsally from the ray 2 precursor cluster (2) to the body seam cell (s), while it is associated with the ray 2 precursor cluster in the wild-type animal (C). For reference, each ray precursor cluster, R1.p, R2.p, the seam cell (s) and the hypodermal syncythium (hyp7) are labeled. Scale bars: 20 µm.