Fig. 1. fgf3 expression in relation to otic placode and otocyst development. (A-H,J-L) Detection of fgf3 mRNA in the developing hindbrain (arrows) of the zebrafish embryo. (A) 90% epiboly. (B) Bud (10 hours). (C) 5 somites (11.75 hours). (D) Flatmount of a 5 somite (11.75 hours) embryo; anterior towards the left. (E) 14 somite (16 hours). (F) 20 somites (19 hours). (G,H) Hybridisation to fgf3 (blue) and krox20 (red) in 4 somite (G) and 14 somite (H) embryos. (I) Detection of hoxb1 (blue) and krox20 (red) in a 12 somite embryo. (J,K) fgf3 (blue) and dlx3 (orange) expression in 3 somite embryos; (K) flatmount. (L) Flatmount showing fgf3 (blue) and pax2.1 (orange) in a 5 somite embryo. (M) Flatmounted 10 somite embryo showing fgf3 (orange) and pax8 (blue). (N) Dorsal view of an embryo at 90% epiboly to show onset of fgf8 expression (arrowhead) in the presumptive r4 territory.