Fig. 5. Loss of early otic placode markers in embryos injected with Fgf3 and Fgf8 morpholinos. Embryos were hybridised for dlx3 (A-H) and pax2.1 (I-P) after incubation to 2-5 somite (A-D and I-L) or 18-21 somite (E-H and M-P) stages after injection with morpholinos. (A,E,I,M) Embryos injected with control Fgf3 morpholino are indistinguishable from uninjected embryos. (B,F,J,N) Embryos injected with Fgf3mo show reduction in level and extent of dlx3 (B,F) and pax2.1 (J,N) expression. (C,G,K,O) Embryos injected with Fgf8mo show reduction in level and extent of dlx3 (C,G) and pax2.1 (K,O) expression. (D,H,L,P) Embryos injected with Fgf8mo and Fgf3mo together show loss of dlx3 (D,H) and pax2.1 (L,P) expression. Arrows indicate position of dlx3- and pax2.1-expressing cells as appropriate.