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Fig. 7. Induction of pax8 in presumptive otic ectoderm is independent of Fgf signals. (A-D) pax8 transcripts are detected in control embryos (A) and embryos injected with Fgf8 morpholino (B), Fgf3 morpholino (C), and Fgf3 and Fgf8 morpholinos together (D), and allowed to develop to 2 somites. (E-H) Treatment with SU5402 does not abolish pax8 expression. (E,F) Embryos treated between 30% epiboly and tailbud. (E) Control. (F) SU5402 treated. (G,H) Embryos treated between 60% epiboly and 2-3 somites. (G) Control. (H) SU5402 treated. Arrows indicate pax8 expression in presumptive otic territory.