Fig. 6. Influence of the foregut and mesoderm on the formation of neural precursors. All embryos viewed laterally. (A-C) ES10 wild type, forkhead loss-of-function and tinman loss-of-function embryos, respectively. The pattern of neuroblasts in b1 was visualized using anti-Hunchback antibody staining (brown) and did not show obvious differences between these groups. (D-F) ES11 wild-type, forkhead loss-of-function, and tinman loss-of-function embryos, respectively. GMCs in the brain were stained using anti-Prospero antibody (brown). (E) In the absence of Forkhead function, the number of GMCs in b1 appeared to be reduced (arrow). (F) A similar reduction in GMCs was not seen in Tinman null embryos. (H) ES11 Hedgehog null embryo, showing a reduction in the number of GMCs in b1 similar to that seen in Forkhead null embryos (arrow).