Fig. 3. Embryonic and neuronal expression of PTP-3. (A-F) Expression of PTP-3B-GFP in enclosure stage embryos. Animals are of genotype juEx189; GFP is visualized by anti-GFP immunostaining (green), animals are also immunostained with MH27 to visualize adherens junctions (red). (A-C) A lateral confocal projection of an embryo prior to epidermal enclosure. The dorsal sheet of epidermal cells is visualised by MH27 staining; PTP-3B::GFP expression is widespread in surface cells; expression in ventral neuroblasts is marked in A (arrowhead). (D-F) A post-gastrulation embryo; medial confocal section showing widespread expression of PTP-3B::GFP at cell surfaces in epidermal, neuronal, pharyngeal, muscle, and endodermal tissue layers. (G) Wild-type L1 larva stained with anti-PTP-3 antibodies and MH27. Nerve ring staining is marked by the arrowhead. (H,I) PTP-3B-GFP expression in L1 (H) and adult (I) (juEx189), stained with anti-GFP antibodies; note intense nerve ring expression (arrowhead in I) and staining in neuronal processes. Scale bar, 10 µm (A-F); 30 µm (G, H); 100 µm (I).