
Fig. 5. cdc-25.1(gf) enhances the pop-1 phenotype. (A) Wild-type number of intestinal nuclei in 300 minute embryo visualized with elt-2::GFP. (B) cdc-25.1(gf) embryo at 300 minutes showing extra intestinal nuclei. (C) pop-1(RNAi) embryos have extra intestinal nuclei due to a MS to E transformation. (D) cdc-25.1(gf); pop-1(RNAi) embryos have twice as many intestinal nuclei as pop-1(RNAi) embryos alone. (E) Laser-mediated cell ablation of the E blastomere in cdc-25.1(gf) animals results in embryos arrested without any intestine as seen by the absence of elt-2::GFP expression. The embryo to the right is an unablated cdc-25.1(gf) mutant embryo, allowed to develop to late embryogenesis.